Journal: Immunology letters

Article Title: A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry.

doi: 10.1016/j.imlet.2014.10.025

Figure Lengend Snippet: Fig. 1. Detection of IL-17A in human neutrophils by IHC. (A) Cyanine3 labelled IL-17A+ cells infiltrate Wolbachia-containing nodules but not Wolbachia-depleted nodules. (B) Counterstaining

Article Snippet: Immunoprecipitation was carried out using 100 g mouse anti-human IL-17A antibody (41802, R&D Systems) and 60 g goat anti-human IL-17A antibody (AF-317-NA, R&D Systems) coupled to 1 mL HiTrap NHS-activated HP columns and AKTAprime Plus low-pressure affinity chromatography system (both from GE Healthcare) using the manufacturer’s protocol.

Techniques:

Journal: Immunology letters

Article Title: A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry.

doi: 10.1016/j.imlet.2014.10.025

Figure Lengend Snippet: Fig. 2. Determination of IL-17A expression by human neutrophils. (A) Western blotting of neutrophil (PMN) cell lysate using goat anti-IL-17A antibody and mouse anti- IL-17A antibody.

Article Snippet: Immunoprecipitation was carried out using 100 g mouse anti-human IL-17A antibody (41802, R&D Systems) and 60 g goat anti-human IL-17A antibody (AF-317-NA, R&D Systems) coupled to 1 mL HiTrap NHS-activated HP columns and AKTAprime Plus low-pressure affinity chromatography system (both from GE Healthcare) using the manufacturer’s protocol.

Techniques: Expressing, Western Blot

Journal: Biomedicines

Article Title: Acetylated Diacylglycerol 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol in Autoimmune Arthritis and Interstitial Lung Disease in SKG Mice

doi: 10.3390/biomedicines9091095

Figure Lengend Snippet: Histologic examination of the lung tissue from SKG mice at 20 weeks after a curdlan injection with/without PLAG treatment. ( A ) Hematoxylin and eosin (upper panels) and Masson’s trichrome (lower panels) staining of the lung tissues. Severe lung inflammations were observed in the curdlan-induced SKG mice and these features were attenuated in the PLAG-treated curdlan-induced SKG mice. ( B ) Opal multiplexed immunofluorescent images and semi-quantitation of the total, GM-CSF + and IL-17A + neutrophil accumulation in the lung tissues. The GM-CSF + neutrophil accumulation was significantly decreased in the PLAG-treated curdlan-induced SKG mice compared with those without PLAG treatment whereas the IL-17A + neutrophil accumulation was not attenuated. The values represent the mean of three independent experiments ± SEM. * p < 0.05. PLAG: acetylated diacylglycerol 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol.

Article Snippet: The slides were again placed in a citrate buffer (pH 6.0), heated in a microwave and incubated with primary rabbit antibodies for IL-17A (Novus Biologicals, Centennial, CO, USA, NBP1-76337, 1:500) for 1 h in a humidified chamber at RT followed by a detection using polymer HRP Ms + Rb.

Techniques: Injection, Staining, Quantitation Assay